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316L ultra thin foil is a typical austenitic Cr-Ni stainless steel alloy. It is particularly well suited for use in marine environments because of its chromium content which provides excellent corrosion resistance to chloride ions. This alloy is also highly resistant to intergranular corrosion and pitting corrosion.
The dimensional accuracy and flatness of ultra thin foil is critical for a number of applications, including optical transmission and imaging in liquid-based microscopy. Foil as thin as this requires a very high degree of rolling expertise to manufacture. As a result, foil can develop slight wrinkles and waves which cause geometric thickness errors that are rarely greater than 2% of the intended value. This is tolerable for most applications, and the increased strength provided by these wrinkles exceeds the error introduced.
Foil can also have pinholes which degrade optical transparency and require care to minimize. Pinholes are caused by dust and other contaminants during manufacturing and inspection. All foil delivered to customers is carefully inspected for pinholes. The maximum allowable light leakage through pinholes in foil over 0.2u thick is 1 part in 105 measured by light transmission. Foil less than 0.2u thick may have 3-6 very small pinholes.
LSFM images of murine pancreas organoids directly seeded into FEP-foil cuvettes and subsequently fixed, labelled and imaged over ten days (240 hours). The image stacks were acquired with an mDSLM using a Epiplan-Neofluar 2.5x/0.06 illumination lens and an N-Achroplan 10x/0.3 detection lens.